PI3KC3-dependent autophagosomes formation pathway is of crucial importance to anti-DHAV activity of Chrysanthemum indicum polysaccharide.
Identifieur interne : 000263 ( Main/Exploration ); précédent : 000262; suivant : 000264PI3KC3-dependent autophagosomes formation pathway is of crucial importance to anti-DHAV activity of Chrysanthemum indicum polysaccharide.
Auteurs : Ke Ming [République populaire de Chine] ; Wenjuan Yuan [République populaire de Chine] ; Yun Chen [République populaire de Chine] ; Hongxu Du [République populaire de Chine] ; Miao He [République populaire de Chine] ; Yuanliang Hu [République populaire de Chine] ; Deyun Wang [République populaire de Chine] ; Yi Wu [République populaire de Chine] ; Jiaguo Liu [République populaire de Chine]Source :
- Carbohydrate polymers [ 1879-1344 ] ; 2019.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Antiviraux (pharmacologie), Autophagosomes (effets des médicaments et des substances chimiques), Autophagosomes (métabolisme), Autophagosomes (virologie), Canards (MeSH), Chrysanthemum (composition chimique), Hépatocytes (virologie), Phosphates phosphatidylinositol (métabolisme), Phosphatidylinositol 3-kinases de classe III (métabolisme), Polyosides (pharmacologie), Réplication virale (effets des médicaments et des substances chimiques), Sirolimus (pharmacologie), Virus de l'hépatite du canard (effets des médicaments et des substances chimiques), Virus de l'hépatite du canard (pathogénicité).
- MESH :
- composition chimique : Chrysanthemum.
- effets des médicaments et des substances chimiques : Autophagosomes, Réplication virale, Virus de l'hépatite du canard.
- métabolisme : Autophagosomes, Phosphates phosphatidylinositol, Phosphatidylinositol 3-kinases de classe III.
- pathogénicité : Virus de l'hépatite du canard.
- pharmacologie : Antiviraux, Polyosides, Sirolimus.
- virologie : Autophagosomes, Hépatocytes.
- Animaux, Canards.
English descriptors
- KwdEn :
- Animals (MeSH), Antiviral Agents (pharmacology), Autophagosomes (drug effects), Autophagosomes (metabolism), Autophagosomes (virology), Chrysanthemum (chemistry), Class III Phosphatidylinositol 3-Kinases (metabolism), Ducks (MeSH), Hepatitis Virus, Duck (drug effects), Hepatitis Virus, Duck (pathogenicity), Hepatocytes (virology), Phosphatidylinositol Phosphates (metabolism), Polysaccharides (pharmacology), Sirolimus (pharmacology), Virus Replication (drug effects).
- MESH :
- chemical , metabolism : Class III Phosphatidylinositol 3-Kinases, Phosphatidylinositol Phosphates.
- chemical , pharmacology : Antiviral Agents, Polysaccharides, Sirolimus.
- chemistry : Chrysanthemum.
- drug effects : Autophagosomes, Hepatitis Virus, Duck, Virus Replication.
- metabolism : Autophagosomes.
- pathogenicity : Hepatitis Virus, Duck.
- virology : Autophagosomes, Hepatocytes.
- Animals, Ducks.
Abstract
We previously reported that Chrysanthemum indicum polysaccharide (CIPS) effectively inhibited the replication of duck hepatitis A virus (DHAV). However, the inhibition mechanisms are still unclear. Autophagy plays important role in virus genomic replication. Therefore, in present study, the effect of autophagy on DHAV genome replication as well as the influence of CIPS on autophagy were studied. qPCR, western blot, and ELISA methods were applied to observe the autophagy and analyze the inhibition mechanisms of CIPS on DHAV. Results showed that DHAV infection increased the expression level of LC3-II and interdicted the degradation of p62. Treating with rapamycin benefited DHAV gene expression level. What's more, DHAV infection and rapamycin treatment also promoted the expression of PI3KC3 and increased the concentration of PI3P. However, CIPS treatment significantly downregulated the expressions of LC3-II and PI3KC3 induced by DHAV and rapamycin, and consequently inhibited autophagosomes formation. As a result, DHAV replication was inhibited.
DOI: 10.1016/j.carbpol.2018.12.035
PubMed: 30658794
Affiliations:
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Le document en format XML
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Electronic address: ylhu@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095</wicri:regionArea><wicri:noRegion>210095</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Deyun" sort="Wang, Deyun" uniqKey="Wang D" first="Deyun" last="Wang">Deyun Wang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: dywang@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095</wicri:regionArea><wicri:noRegion>210095</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Yi" sort="Wu, Yi" uniqKey="Wu Y" first="Yi" last="Wu">Yi Wu</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. 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Electronic address: 2017207037@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095</wicri:regionArea><wicri:noRegion>210095</wicri:noRegion></affiliation></author><author><name sortKey="Yuan, Wenjuan" sort="Yuan, Wenjuan" uniqKey="Yuan W" first="Wenjuan" last="Yuan">Wenjuan Yuan</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2016107097@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095</wicri:regionArea><wicri:noRegion>210095</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Yun" sort="Chen, Yun" uniqKey="Chen Y" first="Yun" last="Chen">Yun Chen</name><affiliation wicri:level="1"><nlm:affiliation>College of Animal Science and Technology, College of Tropical Agriculture and Forestry, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou, 570228, PR China. Electronic address: ychen@hainu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Animal Science and Technology, College of Tropical Agriculture and Forestry, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou, 570228</wicri:regionArea><wicri:noRegion>570228</wicri:noRegion></affiliation></author><author><name sortKey="Du, Hongxu" sort="Du, Hongxu" uniqKey="Du H" first="Hongxu" last="Du">Hongxu Du</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2016207035@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095</wicri:regionArea><wicri:noRegion>210095</wicri:noRegion></affiliation></author><author><name sortKey="He, Miao" sort="He, Miao" uniqKey="He M" first="Miao" last="He">Miao He</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2017107099@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095</wicri:regionArea><wicri:noRegion>210095</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Yuanliang" sort="Hu, Yuanliang" uniqKey="Hu Y" first="Yuanliang" last="Hu">Yuanliang Hu</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: ylhu@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095</wicri:regionArea><wicri:noRegion>210095</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Deyun" sort="Wang, Deyun" uniqKey="Wang D" first="Deyun" last="Wang">Deyun Wang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: dywang@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095</wicri:regionArea><wicri:noRegion>210095</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Yi" sort="Wu, Yi" uniqKey="Wu Y" first="Yi" last="Wu">Yi Wu</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: wuyi2001cn@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095</wicri:regionArea><wicri:noRegion>210095</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Jiaguo" sort="Liu, Jiaguo" uniqKey="Liu J" first="Jiaguo" last="Liu">Jiaguo Liu</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: liujiaguo@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095</wicri:regionArea><wicri:noRegion>210095</wicri:noRegion></affiliation></author></analytic><series><title level="j">Carbohydrate polymers</title><idno type="eISSN">1879-1344</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Antiviral Agents (pharmacology)</term><term>Autophagosomes (drug effects)</term><term>Autophagosomes (metabolism)</term><term>Autophagosomes (virology)</term><term>Chrysanthemum (chemistry)</term><term>Class III Phosphatidylinositol 3-Kinases (metabolism)</term><term>Ducks (MeSH)</term><term>Hepatitis Virus, Duck (drug effects)</term><term>Hepatitis Virus, Duck (pathogenicity)</term><term>Hepatocytes (virology)</term><term>Phosphatidylinositol Phosphates (metabolism)</term><term>Polysaccharides (pharmacology)</term><term>Sirolimus (pharmacology)</term><term>Virus Replication (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Antiviraux (pharmacologie)</term><term>Autophagosomes (effets des médicaments et des substances chimiques)</term><term>Autophagosomes (métabolisme)</term><term>Autophagosomes (virologie)</term><term>Canards (MeSH)</term><term>Chrysanthemum (composition chimique)</term><term>Hépatocytes (virologie)</term><term>Phosphates phosphatidylinositol (métabolisme)</term><term>Phosphatidylinositol 3-kinases de classe III (métabolisme)</term><term>Polyosides (pharmacologie)</term><term>Réplication virale (effets des médicaments et des substances chimiques)</term><term>Sirolimus (pharmacologie)</term><term>Virus de l'hépatite du canard (effets des médicaments et des substances chimiques)</term><term>Virus de l'hépatite du canard (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Class III Phosphatidylinositol 3-Kinases</term><term>Phosphatidylinositol Phosphates</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term><term>Polysaccharides</term><term>Sirolimus</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Chrysanthemum</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Chrysanthemum</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Autophagosomes</term><term>Hepatitis Virus, Duck</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Autophagosomes</term><term>Réplication virale</term><term>Virus de l'hépatite du canard</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Autophagosomes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Autophagosomes</term><term>Phosphates phosphatidylinositol</term><term>Phosphatidylinositol 3-kinases de classe III</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Hepatitis Virus, Duck</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de l'hépatite du canard</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term><term>Polyosides</term><term>Sirolimus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Autophagosomes</term><term>Hépatocytes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Autophagosomes</term><term>Hepatocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Ducks</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Canards</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We previously reported that Chrysanthemum indicum polysaccharide (CIPS) effectively inhibited the replication of duck hepatitis A virus (DHAV). However, the inhibition mechanisms are still unclear. Autophagy plays important role in virus genomic replication. Therefore, in present study, the effect of autophagy on DHAV genome replication as well as the influence of CIPS on autophagy were studied. qPCR, western blot, and ELISA methods were applied to observe the autophagy and analyze the inhibition mechanisms of CIPS on DHAV. Results showed that DHAV infection increased the expression level of LC3-II and interdicted the degradation of p62. Treating with rapamycin benefited DHAV gene expression level. What's more, DHAV infection and rapamycin treatment also promoted the expression of PI3KC3 and increased the concentration of PI3P. However, CIPS treatment significantly downregulated the expressions of LC3-II and PI3KC3 induced by DHAV and rapamycin, and consequently inhibited autophagosomes formation. As a result, DHAV replication was inhibited.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30658794</PMID><DateCompleted><Year>2019</Year><Month>05</Month><Day>13</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1344</ISSN><JournalIssue CitedMedium="Internet"><Volume>208</Volume><PubDate><Year>2019</Year><Month>Mar</Month><Day>15</Day></PubDate></JournalIssue><Title>Carbohydrate polymers</Title><ISOAbbreviation>Carbohydr Polym</ISOAbbreviation></Journal><ArticleTitle>PI3KC3-dependent autophagosomes formation pathway is of crucial importance to anti-DHAV activity of Chrysanthemum indicum polysaccharide.</ArticleTitle><Pagination><MedlinePgn>22-31</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0144-8617(18)31481-4</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.carbpol.2018.12.035</ELocationID><Abstract><AbstractText>We previously reported that Chrysanthemum indicum polysaccharide (CIPS) effectively inhibited the replication of duck hepatitis A virus (DHAV). However, the inhibition mechanisms are still unclear. Autophagy plays important role in virus genomic replication. Therefore, in present study, the effect of autophagy on DHAV genome replication as well as the influence of CIPS on autophagy were studied. qPCR, western blot, and ELISA methods were applied to observe the autophagy and analyze the inhibition mechanisms of CIPS on DHAV. Results showed that DHAV infection increased the expression level of LC3-II and interdicted the degradation of p62. Treating with rapamycin benefited DHAV gene expression level. What's more, DHAV infection and rapamycin treatment also promoted the expression of PI3KC3 and increased the concentration of PI3P. However, CIPS treatment significantly downregulated the expressions of LC3-II and PI3KC3 induced by DHAV and rapamycin, and consequently inhibited autophagosomes formation. As a result, DHAV replication was inhibited.</AbstractText><CopyrightInformation>Copyright © 2018 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ming</LastName><ForeName>Ke</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2017207037@njau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yuan</LastName><ForeName>Wenjuan</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2016107097@njau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yun</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Animal Science and Technology, College of Tropical Agriculture and Forestry, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou, 570228, PR China. Electronic address: ychen@hainu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Du</LastName><ForeName>Hongxu</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2016207035@njau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Miao</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2017107099@njau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Yuanliang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: ylhu@njau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Deyun</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: dywang@njau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Yi</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: wuyi2001cn@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Jiaguo</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: liujiaguo@njau.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>12</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Carbohydr Polym</MedlineTA><NlmUniqueID>8307156</NlmUniqueID><ISSNLinking>0144-8617</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000998">Antiviral Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018129">Phosphatidylinositol Phosphates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.1.137</RegistryNumber><NameOfSubstance UI="D058546">Class III Phosphatidylinositol 3-Kinases</NameOfSubstance></Chemical><Chemical><RegistryNumber>W36ZG6FT64</RegistryNumber><NameOfSubstance UI="D020123">Sirolimus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000998" MajorTopicYN="N">Antiviral Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000071182" MajorTopicYN="N">Autophagosomes</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031202" MajorTopicYN="N">Chrysanthemum</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058546" MajorTopicYN="N">Class III Phosphatidylinositol 3-Kinases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004372" MajorTopicYN="N">Ducks</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004371" MajorTopicYN="N">Hepatitis Virus, Duck</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D022781" MajorTopicYN="N">Hepatocytes</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018129" MajorTopicYN="N">Phosphatidylinositol Phosphates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011134" MajorTopicYN="N">Polysaccharides</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020123" MajorTopicYN="N">Sirolimus</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Autophagosomes</Keyword><Keyword MajorTopicYN="N">Chrysanthemum indicum polysaccharide</Keyword><Keyword MajorTopicYN="N">Duck hepatitis A virus</Keyword><Keyword MajorTopicYN="N">PI3KC3</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>10</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>12</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>12</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>1</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>1</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>5</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30658794</ArticleId><ArticleId IdType="pii">S0144-8617(18)31481-4</ArticleId><ArticleId IdType="doi">10.1016/j.carbpol.2018.12.035</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Ming, Ke" sort="Ming, Ke" uniqKey="Ming K" first="Ke" last="Ming">Ke Ming</name></noRegion><name sortKey="Chen, Yun" sort="Chen, Yun" uniqKey="Chen Y" first="Yun" last="Chen">Yun Chen</name><name sortKey="Du, Hongxu" sort="Du, Hongxu" uniqKey="Du H" first="Hongxu" last="Du">Hongxu Du</name><name sortKey="He, Miao" sort="He, Miao" uniqKey="He M" first="Miao" last="He">Miao He</name><name sortKey="Hu, Yuanliang" sort="Hu, Yuanliang" uniqKey="Hu Y" first="Yuanliang" last="Hu">Yuanliang Hu</name><name sortKey="Liu, Jiaguo" sort="Liu, Jiaguo" uniqKey="Liu J" first="Jiaguo" last="Liu">Jiaguo Liu</name><name sortKey="Wang, Deyun" sort="Wang, Deyun" uniqKey="Wang D" first="Deyun" last="Wang">Deyun Wang</name><name sortKey="Wu, Yi" sort="Wu, Yi" uniqKey="Wu Y" first="Yi" last="Wu">Yi Wu</name><name sortKey="Yuan, Wenjuan" sort="Yuan, Wenjuan" uniqKey="Yuan W" first="Wenjuan" last="Yuan">Wenjuan Yuan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix 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